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Santa Cruz Biotechnology glut4 protein levels
Summary of <t> glucose transporter </t> family members

Glut4 Protein Levels, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 602 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glut4+protein+levels/Glut4/pmc07672939-44-31-11
Average 95 stars, based on 602 article reviews

glut4 protein levels - by Bioz Stars, 2026-09

95/100 stars

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1) Product Images from "Current understanding of glucose transporter 4 expression and functional mechanisms"

Article Title: Current understanding of glucose transporter 4 expression and functional mechanisms

Journal: World Journal of Biological Chemistry

doi: 10.4331/wjbc.v11.i3.76

Figure Legend Snippet: Summary of glucose transporter family members

Techniques Used:

Schematic of insulin-induced translocation of glucose transporter 4 from cytosol to the cell membrane. The binding of insulin to its receptors initiates a signal transduction cascade, which results in the activation of Akt. Akt acts on the glucose transporter 4 (GLUT4) containing vesicles in the cytosol to facilitate their fusion with the cell membrane. When more GLUT4 molecules are present in the membrane, the rate of glucose uptake is elevated. GLUT4: Glucose transporter 4.

Figure Legend Snippet: Schematic of insulin-induced translocation of glucose transporter 4 from cytosol to the cell membrane. The binding of insulin to its receptors initiates a signal transduction cascade, which results in the activation of Akt. Akt acts on the glucose transporter 4 (GLUT4) containing vesicles in the cytosol to facilitate their fusion with the cell membrane. When more GLUT4 molecules are present in the membrane, the rate of glucose uptake is elevated. GLUT4: Glucose transporter 4.

Techniques Used: Translocation Assay, Membrane, Binding Assay, Transduction, Activation Assay

Figure Legend Snippet: Recent studies of glucose transporter 4 expression and translocation in the skeletal muscle

Techniques Used: Expressing, Translocation Assay, Membrane, Control, Western Blot, Muscles, Clinical Proteomics, Real-time Polymerase Chain Reaction, Negative Control, Positive Control, Immunoprecipitation, Isolation, Transgenic Assay, Immunofluorescence, Microscopy, Electron Microscopy, Saline, Injection, In Vitro, Activation Assay, Labeling, Protease Inhibitor

The movement of glucose transporter 4 in adipocytes. Adipose tissue is made of adipocytes. In adipocytes, glucose transporter 4 (GLUT4) can be found in the cell membrane and in the cytosol. The translocation of GLUT4 from cytosolic vesicles to the cell membrane leads to elevated glucose uptake, whereas endocytosis brings GLUT4 back to the cytosol. ( 1): In unstimulated cells, GLUT4 containing membrane portions are internalized in an endocytosis manner to generate vesicles containing GLUT4. GLUT4 vesicles are internalized into early (or sorted) endosomes. They can enter the recovery endoplasmic body, and follow the retrograde pathway to the trans-Golgi network and endoplasmic reticulum-Golgi intermediate compartment or other donor membrane compartments. (2): GLUT4 vesicles derived from the donor membrane structures are secured by tether containing a UBX domain for GLUT4 (TUG) protein. (3): During insulin signal stimulation, GLUT4 vesicles are released and loaded onto the microtubule motor to be transferred to the plasma membrane. The continuous presence of insulin leads to the direct movement of these vesicles to the plasma membrane. (4): GLUT4 vesicles are tethered to motor protein kinesin and other proteins. A stable ternary SNARE complex forms when this occurs. (5): The stable ternary SNARE complex is docked on the target membrane. (6): The docked vesicles rely on SNARE to move to and fuse with the target membrane[ , , ]. GLUT4: Glucose transporter 4.

Figure Legend Snippet: The movement of glucose transporter 4 in adipocytes. Adipose tissue is made of adipocytes. In adipocytes, glucose transporter 4 (GLUT4) can be found in the cell membrane and in the cytosol. The translocation of GLUT4 from cytosolic vesicles to the cell membrane leads to elevated glucose uptake, whereas endocytosis brings GLUT4 back to the cytosol. ( 1): In unstimulated cells, GLUT4 containing membrane portions are internalized in an endocytosis manner to generate vesicles containing GLUT4. GLUT4 vesicles are internalized into early (or sorted) endosomes. They can enter the recovery endoplasmic body, and follow the retrograde pathway to the trans-Golgi network and endoplasmic reticulum-Golgi intermediate compartment or other donor membrane compartments. (2): GLUT4 vesicles derived from the donor membrane structures are secured by tether containing a UBX domain for GLUT4 (TUG) protein. (3): During insulin signal stimulation, GLUT4 vesicles are released and loaded onto the microtubule motor to be transferred to the plasma membrane. The continuous presence of insulin leads to the direct movement of these vesicles to the plasma membrane. (4): GLUT4 vesicles are tethered to motor protein kinesin and other proteins. A stable ternary SNARE complex forms when this occurs. (5): The stable ternary SNARE complex is docked on the target membrane. (6): The docked vesicles rely on SNARE to move to and fuse with the target membrane[ , , ]. GLUT4: Glucose transporter 4.

Techniques Used: Membrane, Translocation Assay, Derivative Assay, Clinical Proteomics

Recent studies of effects of bioactive compounds and chemical drugs on glucose transporter 4 expression and translocation in adipocytes

Figure Legend Snippet: Recent studies of effects of bioactive compounds and chemical drugs on glucose transporter 4 expression and translocation in adipocytes

Techniques Used: Expressing, Translocation Assay, Fluorescence, Immunostaining, Western Blot, Inhibition, Immunoprecipitation, Real-time Polymerase Chain Reaction, Electrophoretic Mobility Shift Assay, Immunofluorescence, In Vitro, In Vivo, Enzyme-linked Immunosorbent Assay, Multiplex Assay, Activity Assay, Knockdown, Produced, Microscopy, Retroviral, Plasmid Preparation, Flow Cytometry

Figure Legend Snippet: Recent studies of mechanisms of glucose transporter 4 expression and translocation in adipocytes

Techniques Used: Expressing, Translocation Assay, Real-time Polymerase Chain Reaction, Electrophoretic Mobility Shift Assay, Immunohistochemistry, Western Blot, Knockdown, Membrane, Transfection, Northern Blot, Nuclear Run-on Assay, Control, Plasmid Preparation, Flow Cytometry, Fluorescence, Microscopy, Over Expression, Isolation, Concentration Assay, Clinical Proteomics, Phospho-proteomics, Immunofluorescence

Figure Legend Snippet: Recent studies of glucose transporter 4 expression and translocation in the heart

Techniques Used: Expressing, Translocation Assay, Membrane, Positive Control, Inhibition, Activation Assay, Isolation, Real-time Polymerase Chain Reaction, Western Blot, Immunofluorescence, Phospho-proteomics, Gene Expression, Control, Immunohistochemistry, Muscles, Saline

Figure Legend Snippet: Recent studies of glucose transporter 4 expression and translocation in the brain

Techniques Used: Expressing, Translocation Assay, Knock-Out, Western Blot, Cell Culture, Clinical Proteomics, Membrane, Activity Assay, Immunocytochemistry, Real-time Polymerase Chain Reaction, Muscles, Control, Phospho-proteomics, Immunofluorescence, Immunohistochemistry, Positive Control, Negative Control, Microscopy, Transgenic Assay

Real-time Polymerase Chain Reaction:

Article Title: Current understanding of glucose transporter 4 expression and functional mechanisms
Article Snippet: Immunohistochemistry, Western blot, real-time PCR. , Brown adipose tissue of Arfrp1 flox/flox and Arfrp1 ad-/-mouse embryos (ED 18.5) and 3T3-L1 cells with knockdown of Arfrp1. Anti-GLUT4 without specifying the vendor (1:1000). , Mice without or with deletion, and 3T3-L1 cells without or with knockdown. , In Arfrp1 ad-/- adipocytes, GLUT4 protein accumulates on the cell membrane rather than staying intracellularly without any change of Slc2a4 mRNA. siRNA-mediated knockdown of Arfrp1 in 3T3-L1 adipocytes has a similar result and increases basal glucose uptake. , [ ] . .. Real-time PCR, Western blot. , 3T3-L1 transfected with Mmu-miR-29a/b/c. Anti-GLUT4 from Santa Cruz Biotechnology (SC-7938). , Cells with or without transfection. , Transfection of miR-29 family members inhibits Slc2a4 mRNA and GLUT4 protein levels in 3T3-L1 cells by inhibiting SPARC expression. , [ ] . .. Northern blot, Western blot, Nuclear run-on assay for the rate of GLUT4 gene transcription. , 3T3-L1 pre and differentiated adipocytes. Rabbit polyclonal GLUT4 antibody form Chemicon. , Treatment groups without or with inhibitors. , Inhibitions of proteasome using Lactacystin and MG132 reduce Slc2a4 mRNA and GLUT4 protein levels in 3T3-L1 cells. , [ ] .

Western Blot:

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Article Title: An association between stress-induced disruption of the hypothalamic-pituitary-adrenal axis and disordered glucose metabolism in an animal model of post-traumatic stress disorder.
Article Snippet: H. Cohen,* N. Kozlovsky,* N. Savion,* M. A. Matar,* U. Loewenthal,* N. Loewenthal, J. Zohar and Z. Kaplan* *Beer-Sheva Mental Health Center, The State of Israel Ministry of Health, Anxiety and Stress Research Unit, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel.. Division of Pediatrics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel.. Division of Psychiatry, The State of Israel Ministry of Health, The Chaim Sheba Medical Center, Sackler Medical School, Tel-Aviv University, Ramat Gan, Israel.

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